Journal: Frontiers in Pharmacology

Article Title: Chemotherapeutic dihydromyricetin with remarkable anti-tumor activity and biosafety for muscle invasive bladder cancer

doi: 10.3389/fphar.2025.1609354

Figure Lengend Snippet: Dihydromyricetin (DHM) inhibited the viability and proliferation of MIBC (T24 and UMUC3) cells in vitro . (a,b) To determine the appropriate DHM concentration for in vitro tests, T24 and UMUC3 cells were co-incubated with DHM at a concentration of 0, 5, 10, 20 and 30 μΜ, respectively. The relatively cell viability were assessed by MTT assay, and the value of 50% inhibiting concentration (IC50) was calculated; (c,d) The MTT proliferation curves of T24 and UMUC3 cells which were incubated with DHM at 0, 5 and 20 μM for consecutive 5 days **P < 0.01, ***P < 0.001.

Article Snippet: The muscle invasive bladder cancer (MIBC) cell lines (T24 and UMUC3) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: In Vitro, Concentration Assay, Incubation, MTT Assay

Journal: Frontiers in Pharmacology

Article Title: Chemotherapeutic dihydromyricetin with remarkable anti-tumor activity and biosafety for muscle invasive bladder cancer

doi: 10.3389/fphar.2025.1609354

Figure Lengend Snippet: Dihydromyricetin (DHM) induced MIBC (T24 and UMUC3) cells cycle arrested in G0/G1 phase. (a,b) Representative flow cytometry images of cell cycle in MIBC cells after treated with DHM for 48 h; (c,d) Quantitative results of cell cycle distribution exhibited a significantly increase in G0/G1 phase cell percentages. **P < 0.01, ***P < 0.001; (e,f) The relative expression of cell cycle related genes (P53, CDK2, CDK4, Cyclin D1, Cyclin E1) in both kinds of cells could be modulated by DHM; (g,h) Western blot images revealed an obvious downregulation of cell cycle proteins, such as CDK2, CDK4, Cyclin D1 and Ki67; (i,j) Representative immunofluorescence (IF) images of Ki67 staining. Blue signal (DAPI): cell nucleus. Red/green signal: Ki67 protein. Scale bar: 100 μm.

Article Snippet: The muscle invasive bladder cancer (MIBC) cell lines (T24 and UMUC3) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Flow Cytometry, Expressing, Western Blot, Immunofluorescence, Staining

Journal: Frontiers in Pharmacology

Article Title: Chemotherapeutic dihydromyricetin with remarkable anti-tumor activity and biosafety for muscle invasive bladder cancer

doi: 10.3389/fphar.2025.1609354

Figure Lengend Snippet: Dihydromyricetin (DHM) promoted MIBC (T24 and UMUC3) cells apoptosis. (a,b) Representative flow cytometry photos of apoptosis in T24 and UMUC3 cells after treated with DHM for 48 h; (c,d) Quantitative results of cell apoptosis rate exhibited an effectively pro-apoptotic activity of DHM. *P < 0.05, **P < 0.01; (e,f) The relative expression of Caspase 3/6/9 in T24 and UMUC3 cells after DHM treatment; (g,h) Western blot images revealed a strong upregulation of apoptosis proteins.

Article Snippet: The muscle invasive bladder cancer (MIBC) cell lines (T24 and UMUC3) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Flow Cytometry, Activity Assay, Expressing, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Chemotherapeutic dihydromyricetin with remarkable anti-tumor activity and biosafety for muscle invasive bladder cancer

doi: 10.3389/fphar.2025.1609354

Figure Lengend Snippet: Dihydromyricetin (DHM) inhibited the migration and survival of MIBC (T24 and UMUC3) cells in vitro . (a) The influence of DHM on MIBC cell survival was evaluated by clonogenic survival assay; (b,c) Quantitative results of cell clone number per filed. ***P < 0.001; (d) Representative images of transwell chamber assay after DHM treatment. (e,f) Transwell chamber assay indicated that the migration ability of T24 and UMUC3 cells was significantly inhibited by DHM. **P < 0.01, ***P < 0.001; (g,h) The anti-migration activity of DHM against T24 cell was investigated by wound healing assay; (i,j) The anti-migration activity of DHM against UMUC3 cell. **P < 0.01, ***P < 0.001.

Article Snippet: The muscle invasive bladder cancer (MIBC) cell lines (T24 and UMUC3) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Migration, In Vitro, Clonogenic Cell Survival Assay, Transwell Chamber Assay, Activity Assay, Wound Healing Assay

Journal: Frontiers in Pharmacology

Article Title: Chemotherapeutic dihydromyricetin with remarkable anti-tumor activity and biosafety for muscle invasive bladder cancer

doi: 10.3389/fphar.2025.1609354

Figure Lengend Snippet: Epithelial-mesenchymal transition (EMT) pathway was blocked by DHM to inhibit MIBC cells migration. (a,b) The relative gene expression of EMT bio-markers (E-cad, N-cad, Vimentin, Snail) in T24 and UMUC3 cells, respectively; (c,d) E-cad protein expression was upregulated, N-cad, Vimentin and Snail protein expression was downregulated. These results indicated that DHM could block EMT pathway, and then cause the migration inhibition of MIBC cells directly.

Article Snippet: The muscle invasive bladder cancer (MIBC) cell lines (T24 and UMUC3) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Migration, Gene Expression, Expressing, Blocking Assay, Inhibition

Journal: American Journal of Translational Research

Article Title: Gene expression profiles to analyze the anticancer and carcinogenic effects of arsenic in bladder cancer

doi:

Figure Lengend Snippet: The expression of DEGs belonging to different intersections. Human bladder cancer cells T24 were treated with 1 μM ATO for 1 h and 6 h. Expression values were calculated using 2-ΔΔCT method and GAPDH as the endogenous reference gene.

Article Snippet: Cell culture and treatment The T24 human bladder cancer cell line was purchased from Procell Co. Ltd., and mycoplasma testing was conducted.

Techniques: Expressing

Journal: American Journal of Translational Research

Article Title: Gene expression profiles to analyze the anticancer and carcinogenic effects of arsenic in bladder cancer

doi:

Figure Lengend Snippet: Expression of putative downstream genes of arsenic binding proteins in each expression matrix (A) or T24 cells examined by real-time PCR (B). Human T24 bladder cancer cells were treated with 1 μM ATO for 1 h and 6 h. Expression values were calculated using the 2-ΔΔCT method and GAPDH as the endogenous reference gene.

Article Snippet: Cell culture and treatment The T24 human bladder cancer cell line was purchased from Procell Co. Ltd., and mycoplasma testing was conducted.

Techniques: Expressing, Binding Assay, Real-time Polymerase Chain Reaction